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BIO 315 Microbiology
Task
These articles, pay attention to the PRNT assay.
Your Experimental Setup section should clearly the methods used to generate the result. Your submission must include the criteria listed below.
(Note: Copying and pasting text from the paper is not allowed. Copying text and presenting it as your own words is plagiarism. Re-write all answers in your own words.)
You are assigned Figure 3BC&D from SARS-CoV-2 Delta Variant Pathogenesis and Host Response in Syrian Hamsters Link to reference:
https://www.mdpi.com/1999-4915/13/9/1773/htm#B20-viruses-13-01773
Only answer questions based on figure 3bcd. Answer these questions:
1. What is the stated goal of the paper?
2. What hypothesis drives the researchers in this paper?
3. Question addressed: What question do the authors want to answer with your assigned figure?
4. Hypothesis: A statement indicating what conclusion the authors expect to see. It is based on previous observations. This may be a plainly written “If [this], then [that]” statement, but not always. There may be more than one hypothesis possible for any figure. This should be written as a testable statement.
5. Null Hypothesis: Null hypotheses specify what should be observed when the hypothesis being tested isn’t correct or supported. “It won’t work” isn’t a null hypothesis. You should indicate what the opposite of the expected conclusion would be.
6. Experimental Setup: Read This Part Carefully This is a condensed version of the experimental design that lets the reader know in a simplified way how the experiment was setup and what observations or measurements were recorded.
There are two parts to this:
a. A picture or cartoon of what the authors did in a step by step manner. The images can be hand-drawn or computer generated, but should have content that is original to you. It may be convenient to grab photos from the internet and string them together, but often images that aren’t yours might not accurately describe what is going on in this specific paper. If you take images from a source, you need to include the source of that site as a url that links to where the image is from. Not in your diagram, but under it. Urls that do not point directly to the image will not be allowed. This is an image, with minimal text. Not a series of text blocks.
b. The text needs to show clear understanding of the procedure, the what materials were used, and how PRNTXX was determined.
c. Things you should include if the information is available:
i. What viruses they used in their PRNT.
ii. A statement (and images) of where the antibodies came from what and what they are expected to bind, down to the epitope, if that is known. iii. What cell line was used. iiii. Name the staining technique
7. Prediction of Hypothesis: This is the predicted results based on observations that would support the hypothesis. If the authors are correct, what is the result?
8. Prediction of Null Hypothesis: This is the predicted results based on observations that would not support the hypothesis. If the authors had been wrong, what would these results look like?
9. Results: This is a brief representation of the results, often in the form of a graph or table. You can include the actual figure form the table, but you need to describe it in your own words. A direct copy and paste of the author’s text is not acceptable.
10. Conclusion: This is an interpretation of the results and an indication of whether or not they support the hypothesis. The conclusion can be quoted from the author, but how it supports the hypothesis should be in your own words.
11. How does this figure fit into the overall hypothesis of the paper?
12. Does this figure help answer the central question of the paper? Describe how it does, or does not.
BIO 315 Microbiology
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