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CEPI0913 Introduction to Nanotechnology
Questions:
- Briefly, what (who, when?) are the origins of cell culture methods?
- What are some types of cells that are routinely cultured?
- List the issues and remedies of basic cell culture.
- What are some disadvantages of 2D cell culture?
- What are self-assembling peptide structures, and what is their interest for cell culture?
- What is meant by cellularizing a nanoscaffold, and what are some means to effect such?
Chapter 7: “Nanoparticle-Based Drug Delivery”
- What is the difference between tumor-specific antigens and tumor-associated antigens?
- What is the difference between active targeting and passive targeting of e.g., tumor cells?
- What are PLAs and how are they designed for controlled release?
- Describe some of the features of penicillin delivered by PACA, including a method to evaluate its effectiveness.
- What are some criteria for nanoparticle based drug delivery systems?
- What is a Kaplan-Meier survival curve, and what is its use?
- What does it mean to derivatize C60 (bucky balls), and what does it enable?
- Describe the transfection assay to assess the uptake of C60 into cells.
- What is the significance of Figure 7.23?
- What are some advantageous features of chitosan for drug delivery?
Chapter 8 “Nanodiagnostics”
- How are most nanofluidic channels fabricated? (Note this is different than microfluidics which are made in plastic by machining or laser cutting.)
- What type of sensor (DNA, immunoassay, cell, metabolite) is shown in Figure 8.4?
- What is a translocation event signal in a nanopore and how is it related to DNA sequencing?
- McDevitt’s Oral Cancer Chip took a picture of captured cells from an oral biopsy. What six criteria were cells evaluated to discern cancer or pre-cancer?
- What is SERS?, how is it used to detect molecules, and why is it more conducive to multiplexing?
- What does in vivo nanodiagnostics accomplish?
Chapter 9 “Government Influence on Nanotechnology”
(We’ll skip this chapter. You can read it at your own leisure, especially if you’re interested in funding.)
Chapter 10: “Future Concepts in Nanomedicine”
- What powers a rotaxane motor, how fast does it turn, how long does it last?
- How do you make the DNA motor of Tan et al open and close?
- What powers J Wang’s nanowire motors? How fast do they go?
- Have ‘nano-gears’ been demonstrated? What do you suppose might be some challenges?
- What is theranostics?
- What are respirocytes?
- What are some nano-based therapies for serious kidney disease?
- The resolution of conventional microscopy/endoscopy are limited by the wavelength of light used? What is one approach to circumvent this? Briefly describe.
CEPI0913 Introduction to Nanotechnology
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