My main question is which specific approach is best to activate the T cells. I cannot use anti-CD3 dynabeads, as macrophages phagocytose them. Consequently, I was planning on using plate bound anti-CD3. However as the macrophages are matured from monocytes, they are already in the well and don’t respond well to detachment and replating, and therefore I think using plate bound will be difficult. Consequently:
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